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Test Cross

subtopicmedium~15 min study9 MCQ

A cross used to determine the genotype of an individual with a dominant phenotype by crossing it with a homozygous recessive parent.

What is Test Cross?

A cross between an individual exhibiting a dominant trait and a homozygous recessive individual to determine the genotype of the former.

Key points

  • Define a test cross.
  • Explain the procedure of a test cross.
  • Interpret the results of a test cross to determine an unknown genotype.
  • Differentiate a test cross from other types of genetic crosses.

Common exam trap

Using a homozygous dominant or heterozygous individual as the tester parent.

Definitions

Term

Test Cross

Meaning

A cross between an individual exhibiting a dominant trait and a homozygous recessive individual to determine the genotype of the former.

Term

Homozygous Recessive

Meaning

An individual having two identical recessive alleles for a particular gene (e.g., 'tt').

Learning objectives

  • Define a test cross.

  • Explain the procedure of a test cross.

  • Interpret the results of a test cross to determine an unknown genotype.

  • Differentiate a test cross from other types of genetic crosses.

Prerequisites

  • Understanding of Mendelian genetics

  • Concepts of dominant and recessive alleles

  • Homozygous and heterozygous genotypes

  • Monohybrid cross

Common mistakes

  • Using a homozygous dominant or heterozygous individual as the tester parent.

  • Incorrectly interpreting the offspring phenotypic ratios.

  • Confusing test cross with back cross or reciprocal cross.

Keywords

  • Test cross

  • Genotype determination

  • Dominant phenotype

  • Homozygous recessive

  • Heterozygous

  • Homozygous dominant

  • Mendelian genetics

  • Punnett square

Practice preview

  • In a test cross, if the individual with the dominant phenotype is heterozygous (e.g., Tt), what will be the phenotypic ratio of the offspring?

    easy

  • What is the primary purpose of a test cross?

    easy

  • Which of the following is always used as the parent with the unknown genotype in a test cross?

    easy